(06-20-2011 11:38 AM)kmacc45 Wrote:  Is your math right dbot?
I thought 1 mg/ml = 1000 mcg

My droppers (pipettes) give me 50 drops per ml, which give me 20 mcg per drop
Correct me if I'm wrong, please
I cut grass for a living, smile.

A drop should be about 50 microliters in volume, with each microliter being associated with one microgram of product. So there should be only about 20 drops per mL. If not, I've been measuring everything wrong; I'll have to test this out.

(06-20-2011 12:07 PM)dbot Wrote:  A drop should be about 50 microliters in volume, with each microliter being associated with one microgram of product. So there should be only about 20 drops per mL. If not, I've been measuring everything wrong; I'll have to test this out.

I'm doing my measurements with pure PA. I fill a 1ml syrine, empty into a cup and pick it up with my pipette. I get 50 drops to a ml.

(06-20-2011 12:07 PM)dbot Wrote:  A drop should be about 50 microliters in volume, with each microliter being associated with one microgram of product. So there should be only about 20 drops per mL. If not, I've been measuring everything wrong; I'll have to test this out.

Pago tells me 40 drops per ml = 25mcg drop...

Whatever, 50, 25 or 20mcg per drop is to high for a blind test.

(06-20-2011 12:19 PM)kmacc45 Wrote:  I'm doing my measurements with pure PA. I fill a 1ml syrine, empty into a cup and pick it up with my pipette. I get 50 drops to a ml.

Ah, okay. Yeah, there should be more drops per mL with alcohol than with water (since lower viscosity = smaller drops). I didn't expect the difference to be that much though.

It should be closer to the volume per drop of water if the sample had DPG added.

(06-20-2011 12:23 PM)dbot Wrote:  Ah, okay. Yeah, there should be more drops per mL with alcohol than with water (since lower viscosity = smaller drops). I didn't expect the difference to be that much though.

It should be closer to the volume per drop of water if the sample had DPG added.

37 drops with water, that give me 27.02 mcg per drop. OK guys, what is our standard so we can all be on the same page? Keep in mind that our droppers must jive too.

I don't think the size of the dropper should matter, only the surface tension, which is affected by temperature, so if you're in hot Florida your drops may be slightly smaller than mine in (currently temperate) Chicago. But I'm not sure why we're getting such different numbers...

I did some testing and determined that I was wrong before in equating viscosity with surface tension. The two appear to be unrelated, which means my numbers have been off for a while.

My tests at 71 degrees came up as such:

1 mL tap water = 20 drops even [50 mcL]

1 mL 96% ethanol = 40 drops average [25 mcL]

1 mL 96% ethanol with 10% FCO & 10% monolaurin = 40 drops average [25 mcL]

1 mL FCO = 50 drops! (I didn't expect that!) [20 mcL]

1 mL ethanol with 20% monolaurin = 40 drops [25 mcL]

I don't have DPG to test, but I expect that it will be about 40 drops per mL, or 25mcg of pheromone per drop. If someone else can test this it would be great.

I'm going to go back to some of my other posts and try to correct my math on the dosages. Need to relabel all my bottles too!

(06-19-2011 5:13 PM)dbot Wrote:  One note: I'm told the samples were distributed in 1mg/mL concentration, which puts each drop at around 50mcgs 25mcgs of pheromone. These tests were conducted using 1-10 drops, with most trials being between 2 and 4 drops, or 100-200mcgs 50-100mcg. So these results represent a moderate to high dose range. The lower range effects we can all suss out together
...

50uG sounds a really high dosage!